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pierce 96 well streptavidin coated microplates  (Thermo Fisher)
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streptavidin coated high capacity microplates  (Thermo Fisher)
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Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 <t>streptavidin</t> Dynabeads or protein G Dynabeads or Streptavidin <t>microplates</t>
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streptavidin-coated microplates 384-well  (Greiner Bio)
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Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 <t>streptavidin</t> Dynabeads or protein G Dynabeads or Streptavidin <t>microplates</t>
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384 well microplates streptavidin-coated 781990  (Greiner Bio)
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Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 <t>streptavidin</t> Dynabeads or protein G Dynabeads or Streptavidin <t>microplates</t>
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streptavidin-coated microplates  (MicroCoat Biotechnologie)
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Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 <t>streptavidin</t> Dynabeads or protein G Dynabeads or Streptavidin <t>microplates</t>
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96-well streptavidin-coated microplate  (Greiner Bio)
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Greiner Bio 96-well streptavidin-coated microplate
Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 <t>streptavidin</t> Dynabeads or protein G Dynabeads or Streptavidin <t>microplates</t>
96 Well Streptavidin Coated Microplate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 streptavidin Dynabeads or protein G Dynabeads or Streptavidin microplates

Journal: Alzheimer's Research & Therapy

Article Title: An improved immunoassay detects Aβ oligomers in human biofluids: their CSF levels rise with tau and phosphotau levels

doi: 10.1186/s13195-025-01802-x

Figure Lengend Snippet: Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 streptavidin Dynabeads or protein G Dynabeads or Streptavidin microplates

Article Snippet: Wells of Streptavidin Coated High-Capacity Microplates (Pierce) were coated with 50 μL of a 6 μg/mL solution of biotinylated 71A1 in Oligomer Aβ Assay Buffer (see Methods) (proprietary, made by EMD Millipore 02–0607-00 specifically for the 71A1/3D6 assay).

Techniques: Biomarker Discovery, Bead-based Assay